Troop E is located on US-67 just North of the city limits of Poplar Bluff in Butler County. Troop E is one of the original six troops within the Highway Patrol. The first Troop E Headquarters in 1931 was located in Sikeston. In 1937, the headquarters was moved to Poplar Bluff. The first Highway Patrol service center was opened in 1973 in Sikeston to serve the citizens of that area. There are approximately 89 uniformed officers, 61 uniformed civilians, and 13 civilian employees assigned to this 13-county Southeast Missouri troop. The Troop E Service Center is located on US-60 approximately one mile west of I-55 in Sikeston.

Counties Served: Bollinger, Butler, Cape Girardeau, Dunklin, Iron, Madison, Mississippi, New Madrid, Pemiscot, Ripley, Scott, Stoddard, and Wayne.